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HP for Mad Lab Siena

21 images Created 30 Oct 2020

Monoclonal Antibody Discovery (MAD) Lab di Fondazione Toscana Life Sciences , tuscany
The MAD Lab research team at TLS Foundation started activities in 2018 thanks to a 2.5 million euros ERC Advanced Grant focused on anti-microbial resistance and has grown quickly thanks to various funding sources received for tackling different health challenges (Shigella, Klebsiella pneumoniae and, more recently, coronavirus SARS-CoV-2). The Lab has consolidated skills in the identification and production of monoclonal antibodies that can be tested in vitro against bacteria and viruses.
MabCo19 Project, started in collaboration with INMI Spallanzani, is focused on the discovery and development of human monoclonal antibodies against coronavirus SARS-CoV-2, from blood of recovering/recovered patients, with the aim of using them for prophylactic/therapeutic purposes and as molecular baits for the discovery of vaccine antigens. The experimental approach followed is called Reverse Vaccinology 2.0, which represents the evolution of the first Reverse Vaccinology strategy optimized by Dr. Rino Rappuoli.

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  • Siena, Mad Lab, Lab Meeting with scientific coordinator Dr Rino Rappuoli
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  • Siena, Mad Lab, Claudia Sala, Senior Scientist at Monoclonal Antibody Discovery (MAD) Lab, Toscana Life Sciences Foundation
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  • Siena, Mad Lab, The well plates in use to do the assays
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  • Siena, Mad Lab, The well plates in use to do the assays
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  • Siena, Mad Lab, Marco Troisi and Anna Kabanova working in the Lab
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  • Siena, Mad Lab, Supernatants recovery by centrifuge
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  • Siena, Mad Lab, Emanuele Andreano using the optical microscope
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  • Siena, Mad Lab, checking cells
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  • Siena, Mad Lab, Cell flasks into the incubator
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  • Emanuele Andreano, researcher at Monoclonal Antibody Discovery (MAD) Lab, Toscana Life Sciences Foundation
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  • Marco Troisi, researcher at Monoclonal Antibody Discovery (MAD) Lab, Toscana Life Sciences Foundation
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  • Fabiola Vacca, researcher at Monoclonal Antibody Discovery (MAD) Lab, Toscana Life Sciences Foundation
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  • Siena, Mad Lab, Fabiola Vacca taking the 3 selected MAbs (Monoclonal Antibodies) stored in the freezer (-80°C)
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  • Siena, Mad Lab, Anna Kabanova, researcher at Monoclonal Antibody Discovery (MAD) Lab, Toscana Life Sciences Foundation
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  • Siena, Mad Lab, working at the chromatography system for recombinant antibody purification
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  • Siena, Mad Lab, Lab equipment
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  • Siena, Mad Lab, Claudia Sala sticking her hands in to manipulate the equipment in the big cabinets with ventilation hood
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  • Siena, Mad Lab, Ida Paciello using the well plate to do the assays
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  • Siena, Mad Lab, The 3D bioprinter in use
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  • Siena, Mad Lab, from left Emanuele Andreano, Claudia Sala, Anna Kabanova and Marco Troisi
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  • Siena, Mad Lab, Claudia Sala, Senior Scientist at Monoclonal Antibody Discovery (MAD) Lab, Toscana Life Sciences Foundation
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Alberto Bernasconi

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